Rousset, Matthieu published the artcileMammalian Brain Ca2+ Channel Activity Transplanted into Xenopus laevis Oocytes, Quality Control of 21829-25-4, the main research area is calcium channel Xenopus laevis oocyte mammalian brain; CaV2 Ca2+ channels; channelopathies; membrane microtransplantation; voltage clamp.
Several mutations on neuronal voltage-gated Ca2+ channels (VGCC) have been shown to cause neurol. disorders and contribute to the initiation of epileptic seizures, migraines, or cerebellar degeneration. Anal. of the functional consequences of these mutations mainly uses heterologously expressed mutated channels or transgenic mice which mimic these pathologies, since direct electrophysiol. approaches on brain samples are not easily feasible. We demonstrate that mammalian voltage-gated Ca2+ channels from membrane preparation can be microtransplanted into Xenopus oocytes and can conserve their activity. This method, originally described to study the alteration of GABA receptors in human brain samples, allows the recording of the activity of membrane receptors and channels with their native post-translational processing, membrane environment, and regulatory subunits. The use of hippocampal, cerebellar, or cardiac membrane preparation displayed different efficacy for transplanted Ca2+ channel activity. This technique, now extended to the recording of Ca2+ channel activity, may therefore be useful in order to analyze the calcium signature of membrane preparations from unfixed human brain samples or normal and transgenic mice.
Membranes (Basel, Switzerland) published new progress about Brain. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Quality Control of 21829-25-4.