2-Pyridineformamide N(4)-ring incorporated thiosemicarbazones inhibit MCF-7 cells by inhibiting JNK pathway was written by Shakya, Bhushan;Shahi, Nerina;Ahmad, Faiz;Yadav, Paras Nath;Pokharel, Yub Raj. And the article was included in Bioorganic & Medicinal Chemistry Letters in 2019.Application of 1620-76-4 This article mentions the following:
In an effort to develop a more potent anticancer therapeutic agent, a series of 2-pyridineformamide thiosemicarbazones (I) (R = H, 4-CH3, 5-F, 6-CH3; heterocycle = pyrrolidine, piperidine, morpholine, thiomorpholine, azepane, 1-(2-pyridinyl)-piperazine) have been synthesized and evaluated for their anti-cancer activities against the cancer cells MCF-7 (breast cancer cell line), A-431 and A375 (epidermoid carcinoma cell line), and HeLa (cervical cancer cell line) using MTT assay. All these 2-pyridineformamide thiosemicarbazones exhibited anti-proliferative activities towards these cell lines. 5FAmPyrr(II) possess most profound effects against MCF-7 cells with IC50 of 0.9 μM. In flow cytometry using Propidium Iodide, II was found to induce cell death significantly in a dose dependent manner (100 nM-3 μM) and inhibited colony formation of MCF-7 cells. This compound induced pro-apoptotic protein Bax and inhibited anti apoptotic protein Bcl-2 as well as both c-Jun and Jun N-terminal kinase (abbreviated as JNK) in concentration dependent manner. Further pro-caspase 3 and PARP were inhibited by II at concentration of 3 μM. The results suggest that II exhibit anticancer potency and induced cell death by inhibiting MAPK signaling and inducing intrinsic apoptotic pathway. All these indicate that 2-pyridineformamide thiosemicarbazones could be developed as future therapeutics agents to treat cancer. In the experiment, the researchers used many compounds, for example, 4-Methylpicolinonitrile (cas: 1620-76-4Application of 1620-76-4).
4-Methylpicolinonitrile (cas: 1620-76-4) belongs to pyridine derivatives. The pyridine ring occurs in many important compounds, including agrochemicals, pharmaceuticals, and vitamins. Reduced pyridines, namely tetrahydropyridines, dihydropyridines and piperidines, are found in numerous natural and synthetic compounds. The synthesis and reactivity of these compounds have often been driven by the fact many of these compounds have interesting and unique pharmacological properties. Application of 1620-76-4