Safety of 3-Cyanopyridine《High-throughput screening of a nicotinate dehydrogenase producing Pseudomonas putida mutant for efficient biosynthesis of 6-hydroxynicotinic acid》 was published in 2021. The authors were Shang, Yu-Ting;Qin, Jiufu;Gong, Jin-Song;Wang, Zi-Kai;Li, Heng;Li, Hui;Shi, Jin-Song;Xu, Zheng-Hong, and the article was included in《Molecular Catalysis》. The author mentioned the following in the article:
A nicotinate dehydrogenase producing strain was isolated and identified to be Pseudomonas putida, which could bio-transform nicotinic acid into 6-hydroxynicotinic acid. The activity of nicotinate dehydrogenase towards nicotinic acid could reach up to 1.11 U/mL and the yield of 6-hydroxynicotinic acid accumulated to 176 g/L within 30 h with a space time yield of 1.36 g/(g·h). In order to further improve its catalytic potential, a mutant library was constructed through atm. and room temperature plasma (ARTP) and rapidly screened by a high-throughput screening strategy, which obtained a mutant strain mut-5 with activity of 2.68 U/mL. Via developing a nitrilase-nicotinate dehydrogenase enzymic cascade system, 54.5 g/L 6-hydroxynicotinic acid could be obtained within 585 min with 3-cyanopyridine as the substrate, which would further reduce the production costs. The results indicated that the nicotinate dehydrogenase (sep. or combining with nitrilase) could serve as a superior catalyst for 6-hydroxynicotinic acid production To our knowledge, the nicotinate dehydrogenase activity reported in this study was the highest level in literature to date. The experimental procedure involved many compounds, such as 3-Cyanopyridine (cas: 100-54-9) .
3-Cyanopyridine(cas: 100-54-9) has been shown to have a number of pharmacological effects: it inhibits the production of prostaglandin E2 and nitric oxide in congestive heart failure patients; it prevents the formation of diazonium salt from benzene and nitrogen dioxide; it inhibits the growth of tumor cell lines; and it protects mice from radiation injury by scavenging reactive oxygen species. Safety of 3-Cyanopyridine