Singh, Malvinder P.; Joseph, Tomi; Kumar, Surat; Bathini, Yadagiri; Lown, J. William published the artcile< Synthesis and sequence-specific DNA binding of a topoisomerase inhibitory analog of Hoechst 33258 designed for altered base and sequence recognition>, COA of Formula: C6H7N3O2, the main research area is topoisomerase inhibitor DNA binding preparation; DNA binding Hoechst 33258 analog sequence; safety benzoyl peroxide explosive; health hazard methoxymethyl chloride nitrobenzene.
The preparation and DNA binding characteristics of a structural analog of Hoechst 33258 bearing 2 pyridine N atoms are described. The 1H NMR signals of the complex formed between the new ligand I and decadeoxyribonucleotide d(CATGGCCATG)2 were assigned by employing 1- and 2-dimensional NMR techniques. Intermol. nuclear Overhauser effects (NOE) between the ligand and the DNA receptor fragment confirm that the ligand binds in the minor groove of the DNA, interacting with the centrally located 5′-GGCCA segment. In contrast to the steric interaction between the benzimidazole rings of the parent Hoechst 33258 mol. and the guanine 2-NH2 groups, which renders it G·C avoiding and thus A·T base pair preferring, the ligand I described here overcomes these unfavorable interactions and instead exhibits a marked preference for G·C base pairs. This behavior appears to arise from addnl. stabilization due to H-bonding with the guanine 2-NH2 groups. Although a ligand-induced distortion at the binding site is qual. assessable, the overall B-type conformation of the DNA fragment is retained upon complexation. The structural conclusions drawn from the NOE-NMR evidence were confirmed by mol. mechanics and mol. modeling studies. Safety in the use of methoxymethyl chloride and nitrobenzene, suspected toxins and benzyl peroxide, a potential explosive, is emphasized.
Chemical Research in Toxicology published new progress about DNA, complexes Role: BIOL (Biological Study). 21901-29-1 belongs to class pyridine-derivatives, and the molecular formula is C6H7N3O2, COA of Formula: C6H7N3O2.