Song, Min-Young; Hwang, Ji Yeon; Bae, Eun Ji; Kim, Saesbyeol; Kang, Hye-Min; Kim, Yong Jun; Park, Chan; Park, Kang-Sik published the artcile< Tyrosine phosphorylation of the Kv2.1 channel contributes to injury in brain ischemia>, Recommanded Product: 1,2-Di(pyridin-2-yl)disulfane, the main research area is tyrosine phosphorylation potassium channel brain ischemia injury; Kv2.1; brain ischemia; oxidative stress; tyrosine phosphorylation.
In brain ischemia, oxidative stress induces neuronal apoptosis, which is mediated by increased activity of the voltage-gated K+ channel Kv2.1 and results in an efflux of intracellular K+. The mol. mechanisms underlying the regulation of Kv2.1 and its activity during brain ischemia are not yet fully understood. Here this study provides evidence that oxidant-induced apoptosis resulting from brain ischemia promotes rapid tyrosine phosphorylation of Kv2.1. When the tyrosine phosphorylation sites Y124, Y686, and Y810 on the Kv2.1 channel are mutated to non-phosphorylatable residues, PARP-1 cleavage levels decrease, indicating suppression of neuronal cell death. The tyrosine residue Y810 on Kv2.1 was a major phosphorylation site. In fact, cells mutated Y810 were more viable in our study than were wild-type cells, suggesting an important role for this site during ischemic neuronal injury. In an animal model, tyrosine phosphorylation of Kv2.1 increased after ischemic brain injury, with an observable sustained increase for at least 2 h after reperfusion. These results demonstrate that tyrosine phosphorylation of the Kv2.1 channel in the brain may play a critical role in regulating neuronal ischemia and is therefore a potential therapeutic target in patients with brain ischemia.
International Journal of Molecular Sciences published new progress about Apoptosis. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Recommanded Product: 1,2-Di(pyridin-2-yl)disulfane.