Stapleton, David; Gao, Guang; Michell, Belinda J.; Widmer, Jane; Mitchelhill, Ken; Teh, Trazel; House, Colin M.; Witters, Lee A.; Kemp, Bruce E. published the artcile< Mammalian 5'-AMP-activated protein kinase non-catalytic subunits are homologs of proteins that interact with yeast Snf1 protein kinase>, Name: 3-Bromo-1H-pyrrolo[3,2-c]pyridine, the main research area is AMP activated protein kinase subunit sequence; rat AMP activated protein kinase subunit; pig AMP activated protein kinase subunit; Snf1 kinase interacting protein homolog mammal; yeast CAT3 Snf4p homolog rat pig; Sip1p GAL38 yeast homolog rat pig.
The 5′-AMP-activated protein kinase is responsible for the regulation of fatty acid synthesis by phosphorylation and inactivation of acetyl-CoA carboxylase. The porcine liver 5′-AMP-activated protein kinase 63-kDa catalytic subunit co-purifies 14,000-fold with a 38- and 40-kDa protein (Mitchelhill, K. I. et al. (1994) J. Biol. Chem. 269, 2361-2364). The 63-kDa subunit is homologous to the Saccharomyces cerevisiae Snf1 protein kinase, which regulates gene expression during glucose derepression. Peptide amino acid and polymerase chain reaction-derived partial cDNA sequences of both the pig and rat liver enzymes show that the 38-kDa protein is homologous to Snf4p (CAT3) and that the 40-kDa protein is homologous to the Sip1p/Spm/GAL83 family of Snf1p interacting proteins. Sucrose d. gradient and crosslinking experiments with purified 5′-AMP-activated protein kinase suggest that both the 38- and 40- kDa proteins associate tightly with the 63-kDa catalytic polypeptide in either a heterotrimeric complex or in dimeric complexes. The 40-kDa subunit is autophosphorylated within the 63-kDa subunit complex. The sequence relationships between the mammalian 5′-AMP-activated protein kinase and yeast Snf1p extend to the subunit proteins consistent with conservation of the functional roles of these polypeptides in cellular regulation by this family of metabolite-sensing protein kinases.
Journal of Biological Chemistry published new progress about Phosphorylation, autophosphorylation. 23612-36-4 belongs to class pyridine-derivatives, and the molecular formula is C7H5BrN2, Name: 3-Bromo-1H-pyrrolo[3,2-c]pyridine.