Vallejo, Luis Felipe published the artcileRenaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli, COA of Formula: C5H5NO3S, the publication is Journal of Biotechnology (2002), 94(2), 185-194, database is CAplus and MEDLINE.
Escherichia coli was genetically engineered to produce recombinant human bone morphogenetic protein-2 (rhBMP-2) in a non-active aggregated form using a temperature-inducible expression system. High concentrations of both biomass (75 g cell dry weight per L of culture broth) and inactive rhBMP-2 (8.6 g l-1) were obtained by applying a high-cell-d. cultivation procedure. After washing and solubilizing the inclusion bodies, rhBMP-2 was refolded and dimerized at concentrations up to 100 mg l-1 by means of a simple dilution method with yields exceeding 50%. Finally, a one-step purification procedure based on affinity chromatog. was implemented to isolate the rhBMP-2 dimer. With the established renaturation and purification protocols, yields of more than 10 mg rhBMP-2 dimer per g cell dry weight were obtained corresponding to 750 mg rhBMP-2 dimer per L of culture broth. The purified rhBMP-2 dimer showed biol. activity equivalent to CHO produced rhBMP-2 as tested by the induction of alk. phosphatase activity in C2C12 cells.
Journal of Biotechnology published new progress about 636-73-7. 636-73-7 belongs to pyridine-derivatives, auxiliary class Pyridine,Sulfonic acid, name is Pyridine-3-sulfonic acid, and the molecular formula is C4H3Cl2N3, COA of Formula: C5H5NO3S.
Referemce:
https://en.wikipedia.org/wiki/Pyridine,
Pyridine | C5H5N – PubChem