Chitin biopolymer mediates self-sufficient biocatalyst of pyridoxal 5′-phosphate and L-lysine decarboxylase was written by Wei, Guoguang;Chen, Yan;Zhou, Ning;Lu, Qiuhao;Xu, Sheng;Zhang, Alei;Chen, Kequan;Ouyang, Pingkai. And the article was included in Chemical Engineering Journal (Amsterdam, Netherlands) in 2022.Electric Literature of C8H10NO6P The following contents are mentioned in the article:
Enzyme cofactors are essential in biocatalysis for many enzymes. However, their high com. cost limits the industrial application of cofactor-dependent enzymes. We overcame this challenge by constructing a self-sufficient biocatalyst that co-immobilized pyridoxal 5′-phosphate (PLP) and L-lysine decarboxylase (LDC) using natural polymer chitin as the functional material. First, degree of deacetylation (DDA) of chitin was regulated to enhance PLP adsorption and immobilization. Then, the enzyme was immobilized via fusion of a chitin-binding domain. Chitin with 36% DDA efficiently co-immobilized LDC and PLP. Laser scanning confocal microscope and scanning electron microscope proved that the LDC and PLP were immobilized on the chitin. The self-sufficient biocatalyst retained over 55% of its original activity after five cycles. The proposed strategy provides a new way to achieve co-immobilization of PLP-dependent enzymes. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Electric Literature of C8H10NO6P).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. The ring atoms in the pyridine molecule are sp2-hybridized. The nitrogen is involved in the 蟺-bonding aromatic system using its unhybridized p orbital. The lone pair is in an sp2 orbital, projecting outward from the ring in the same plane as the 蟽 bonds. Pyridine, its benzo and pyridine-based compounds play diverse roles in organic chemistry. Pyridine-based materials are valued for their optical and physical properties as well as their medical potential. Electric Literature of C8H10NO6P