Chitin biopolymer mediates self-sufficient biocatalyst of pyridoxal 5′-phosphate and L-lysine decarboxylase was written by Wei, Guoguang;Chen, Yan;Zhou, Ning;Lu, Qiuhao;Xu, Sheng;Zhang, Alei;Chen, Kequan;Ouyang, Pingkai. And the article was included in Chemical Engineering Journal (Amsterdam, Netherlands) in 2022.Related Products of 54-47-7 The following contents are mentioned in the article:
Enzyme cofactors are essential in biocatalysis for many enzymes. However, their high com. cost limits the industrial application of cofactor-dependent enzymes. We overcame this challenge by constructing a self-sufficient biocatalyst that co-immobilized pyridoxal 5′-phosphate (PLP) and L-lysine decarboxylase (LDC) using natural polymer chitin as the functional material. First, degree of deacetylation (DDA) of chitin was regulated to enhance PLP adsorption and immobilization. Then, the enzyme was immobilized via fusion of a chitin-binding domain. Chitin with 36% DDA efficiently co-immobilized LDC and PLP. Laser scanning confocal microscope and scanning electron microscope proved that the LDC and PLP were immobilized on the chitin. The self-sufficient biocatalyst retained over 55% of its original activity after five cycles. The proposed strategy provides a new way to achieve co-immobilization of PLP-dependent enzymes. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Related Products of 54-47-7).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridine has a dipole moment and a weaker resonant stabilization than benzene (resonance energy 117 kJ路mol鈭? in pyridine vs. 150 kJ路mol鈭? in benzene). One of the examples of pyridines is the well-known alkaloid lithoprimidine, which is an A3 adenosine receptor antagonist and N,N-dimethylaminopyridine (DMAP) analog, commonly used in organic synthesis.Related Products of 54-47-7