Wisniewski, Jacek R. published the artcile< Filter Aided Sample Preparation - A tutorial>, Electric Literature of 2127-03-9, the main research area is filter aided sample preparation proteomic ultrafiltration; Detergent removal; Filter Aided Sample Preparation; Lysate preparation; Multi enzyme digestion filter aided sample preparation; Protein digestion conditions; Proteomic sample preparation.
Filter Aided Sample Preparation (FASP) is a widely used protein processing technique in “”bottom-up”” proteomics. Its popularity reflects the key features of the method: its applicability to a variety of sample types and the high quality of the released peptides. Successful application of FASP requires optimized properties of sample lysate and its amount, use of ultrafiltration units with membranes having large mol. mass cut-offs and well selected conditions for protein digestion. In contrast to the majority of sample preparation methods, FASP allows digestion of proteins with a variety of enzymes and a straightforward monitoring of protein-to-peptide conversion. A unique feature of FASP is the possibility to cleave proteins in a consecutive way using several proteases and to sep. peptide fractions. Understanding principles of the method gives guidance in applying FASP to different types of samples in optimization of conditions of the FASP-workflow.
Analytica Chimica Acta published new progress about Critical micelle concentration. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Electric Literature of 2127-03-9.