Yamazaki, Kei published the artcileHepatocyte growth factor exhibits anti-fibrotic effects in an in vitro model of nifedipineinduced gingival overgrowth, Related Products of pyridine-derivatives, the main research area is gingiva nifedipine hepatocyte growth factor antifibrotic; drug-induced gingival overgrowth; hepatocyte growth factor; nifedipine.
Purpose: The aim of this study was to establish an in vitro model of nifedipine-induced gingival overgrowth and characterize the anti-fibrotic effect of hepatocyte growth factor (HGF) using this model. Methods: Human gingival fibroblasts were cultured-treated with 0.1, 1, or 10μg/mL nifedipine or 10 ng/mL IL-1β + 0.1, 1, or 10μg/mL nifedipine (0.1N, 1N, 10N, IL + 0.1N, IL + 1N, IL + 10N). Cell proliferation and levels of type I collagen, TGF-β1, CCN2/CTGF, and α-SMA were measured 48 h after the simultaneous addition of 10 and 50 ng/mL HGF (10 and 50HGF) along with IL-1β and nifedipine. Type I collagen was measured after administration of anti-HGF neutralizing antibody. Results: Significant increases in type I collagen, TGF-β1, and CCN2/ CTGF were observed after treatment in the 1N and IL + 0.1N groups. Levels of type I collagen and CCN2/CTGF differed significantly between the IL + 0.1N group and the IL + 0.1N + 50HGF group. Production of type I collagen increased significantly following addition of anti-HGF antibody. Conclusion: This study demonstrated the establishment of an in vitro model of nifedipine-induced gingival overgrowth by showing increased collagen levels. Experiments using this model suggested that HGF exerts anti-fibrotic effects.
Journal of Oral Science published new progress about Antifibrotic agents. 21829-25-4 belongs to class pyridine-derivatives, name is Dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate, and the molecular formula is C17H18N2O6, Related Products of pyridine-derivatives.