Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli was written by Yang, Shih-Chen;Ting, Wan-Wen;Ng, I-Son. And the article was included in Biochemical Engineering Journal in 2022.Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate The following contents are mentioned in the article:
The rising awareness of environmental protection has triggered bio-based materials to replace the traditional petrochem. plastics. Putrescine as 1,4-diaminobutane is an important monomer of polyamide (PA) and uses in the sustainable chem. industry. Herein, a time-effective whole cell bioconversion of L-arginine to putrescine was developed, which has applied the key enzymes (i.e., SpeA and SpeB) from the arginine decarboxylase (ADC) pathway. The synergetic collaboration of both enzymes was examined from the different combination of plasmids among 4 Escherichia coli chassis. The optimal reaction condition was at pH 9 with 1 mM pyridoxal-5â?phosphate (PLP) and 10 mM magnesium, thus 90% conversion was obtained using an all-in-one plasmid with equal protein of SpeA and SpeB in BL21(DE3). The enzymic kinetics demonstrated the higher kcat of SpeA (1212 s-1) than that of SpeB (418 s-1), while severe inhibition of putrescine on SpeA (KI = 8.61 mM), thus it was disadvantage using the surface display of enzyme. To prevent the feedback-inhibition by product, a 2-step enzymic reaction with cold treatment was conducted. Finally, the putrescine was achieved 17.1 g/L with the productivity of 8.56 g/L/h under 86% conversion of 50 g/L -arginine-HCl, which is an effective approach to obtain high putrescine titer. This study involved multiple reactions and reactants, such as (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate).
(4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate (cas: 54-47-7) belongs to pyridine derivatives. Pyridines are an important class of heterocycles and occur in polysubstituted forms in many naturally occurring biologically active compounds, drug molecules and chiral ligands. Pyridine groups exist in countless molecules, and their applications include catalysis, drug design, molecular recognition, and natural product synthesis.Recommanded Product: (4-Formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate