Yi, Sijia; Kim, Sun-Young; Vincent, Michael P.; Yuk, Simseok A.; Bobbala, Sharan; Du, Fanfan; Scott, Evan Alexander published the artcile< Dendritic peptide-conjugated polymeric nanovectors for non-toxic delivery of plasmid DNA and enhanced non-viral transfection of immune cells>, COA of Formula: C10H8N2S2, the main research area is dendritic peptide polymeric nanovector toxic delivery plasmid DNA; plasmid DNA immune cell nonviral transfection; Biological sciences; Biotechnology; Drug delivery system; Health sciences; Immunology.
Plasmid DNA (pDNA) transfection is advantageous for gene therapies requiring larger genetic elements, including “”all-in-one”” CRISPR/Cas9 plasmids, but is limited by toxicity as well as poor intracellular release and transfection efficiency in immune cell populations. Here, we developed a synthetic non-viral gene delivery platform composed of poly(ethylene glycol)-b-poly(propylene sulfide) copolymers linked to a cationic dendritic peptide (DP) via a reduceable bond, PEG-b-PPS-ss-DP (PPDP). A library of self-assembling PPDP polymers was synthesized and screened to identify optimal constructs capable of transfecting macrophages with small (pCMV-DsRed, 4.6 kb) and large (pL-CRISPR.EFS.tRFP, 11.7 kb) plasmids. The optimized PPDP construct transfected macrophages, fibroblasts, dendritic cells, and T cells more efficiently and with less toxicity than a com. Lipo2K reagent, regardless of pDNA size and under standard culture conditions in the presence of serum. The PPDP technol. described herein is a stimuli-responsive polymeric nanovector that can be leveraged to meet diverse challenges in gene delivery.
iScience published new progress about Blood serum. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, COA of Formula: C10H8N2S2.