Zhan, Yu-Rong; Yu, Qing-Ying; Zhang, Ji; Liu, Yan-Hong; Xiao, Ya-Ping; Zhang, Ju-Hui; He, Xi; Yu, Xiao-Qi published the artcile< Glutathione modified low molecular weight PEI for highly improved gene transfection ability and biocompatibility>, Category: pyridine-derivatives, the main research area is glutathione PEI gene transfection biocompatibility.
The efficient delivery of therapeutic genes remains a major challenge in realizing a feasible gene-based treatment. Herein, a versatile oligopeptide, glutathione, was introduced to construct novel non-viral cationic gene vectors. Reduced/oxidized forms of glutathione (GSH/GSSG) and relevant amino acids (Glu, Cys, and Gly) were used to modify low mol. weight PEI through surface modification or crosslinking. These polymers could bind well and condense DNA into spherical nanoparticles, which were stable in the presence of serum. The disulfide bonds within the crosslinked polymer GSSG-PEI may facilitate polymer degradation and DNA release under a reductive environment. In vitro transfection experiments reveal that the modification could largely improve the gene transfection efficiency of low mol. weight PEI, especially in the presence of serum. In HeLa cells, GSSG-PEI could even give up to 150 times higher efficiency than PEI 25 kDa. TEM and serum concentration effect assay also demonstrate the good serum tolerance of the polymers. Flow cytometry results show that GSSG-PEI might induce cellular uptake with higher efficiency than PEI 25 kDa, especially in the presence of serum. Results reveal that GSSG is a good candidate for the crosslinking of small cationic mols. to form polymeric gene vectors with improved transfection efficiency and biocompatibility.
New Journal of Chemistry published new progress about Biocompatibility. 2127-03-9 belongs to class pyridine-derivatives, and the molecular formula is C10H8N2S2, Category: pyridine-derivatives.