Zhong, Jia-Yu; Cui, Xing-Jun; Zhan, Jun-Kun; Wang, Yan-Jiao; Li, Shuang; Lin, Xiao; Xiang, Qun-Yan; Ni, Yu-Qing; Liu, Le; Liu, You-Shuo published the artcile< LncRNA-ES3 inhibition by Bhlhe40 is involved in high glucose-induced calcification/senescence of vascular smooth muscle cells>, Category: pyridine-derivatives, the main research area is lncRNA Bhlhe40 glucose calcification senescence vascular smooth muscle cell; Bhlhe40; VSMC calcification/senescence; diabetes; lncRNA-ES3; microRNA; vascular aging.
Long noncoding RNAs (lncRNAs) have been investigated as novel regulatory mols. involved in diverse biol. processes. Our previous study demonstrated that lncRNA-ES3 is associated with the high glucose-induced calcification/senescence of human aortic vascular smooth muscle cells (HA-VSMCs). However, the mechanism of lncRNA-ES3 in vascular calcification/aging remained largely unknown. Here, we report that the expression of basic helix-loop-helix family member e40 (Bhlhe40) was decreased significantly in HA-VSMCs treated with high glucose, whereas the expression of basic leucine zipper transcription factor (BATF) was increased. Overexpression of Bhlhe40 and inhibition of BATF alleviated calcification/senescence of HA-VSMCs, as confirmed by Alizarin Red S staining and the presence of senescence-associated β-galactosidase-pos. cells. Moreover, we identified that Bhlhe40 regulates lncRNA-ES3 in HA-VSMCs by binding to the promoter region of the lncRNA-ES3 gene (LINC00458). Upregulation or inhibition of lncRNA-ES3 expression significantly promoted or reduced calcification/senescence of HA-VSMCs, resp. Addnl., we identified that lncRNA-ES3 functions in this process by suppressing the expression of miR-95-5p, miR-6776-5p, miR-3620-5p, and miR-4747-5p. The results demonstrate that lncRNA-ES3 triggers gene silencing of multiple miRNAs by binding to Bhlhe40, leading to calcification/senescence of VSMCs. Our findings suggest that pharmacol. interventions targeting lncRNA-ES3 may be therapeutically beneficial in ameliorating vascular calcification/aging.
Annals of the New York Academy of Sciences published new progress about Animal gene Role: BSU (Biological Study, Unclassified), PRP (Properties), BIOL (Biological Study) (CEBPE). 123-03-5 belongs to class pyridine-derivatives, and the molecular formula is C21H38ClN, Category: pyridine-derivatives.