4-Dimensional observation ER-mitochondria interaction in living cells under nanoscopy by a stable pyridium salt as biosensor was written by Zhu, Fenfang;Yang, Zhenghui;Wang, Fei;Li, Dandan;Cao, Hongzhi;Tian, Yupeng;Tian, Xiaohe. And the article was included in Sensors and Actuators, B: Chemical in 2020.Reference of 65350-59-6 This article mentions the following:
Intracellular membrane-bounded organelles are phys. associated and play critical roles in many physiol. processes. However, real-time monitoring of their interaction at the nanoscale in three-dimension (xyz-t or 4D imaging) remains considerable difficulties due to limitations of the current super-resolution probe toolbox. Here, we developed a novel cell-permeable pyridium salt derivative (TpsPym) with red-emission and large Stock-shift. Interestingly, TpsPym demonstrated a high specificity to mitochondria and endoplasmic reticulum (ER) with different fluorescent intensity. This offered the possibility to utilize this probe to label dual-organelles (ER-mitochondria) under stimulated emission depletion nanoscopy (STED). We successfully monitored the dynamic interactions in real-time between mitochondria and ER in three dimension during starvation-induced autophagy. It suggested ER-mitochondria contacting area might play an essential factor during autophagic cell death (ACD). Our study not only expanding the nanoscopic probe kit but also provide new understanding for ER-mitochondrial interaction at the nanoscale during correlated physiol. conditions. In the experiment, the researchers used many compounds, for example, 1-Butyl-4-methylpyridin-1-ium bromide (cas: 65350-59-6Reference of 65350-59-6).
1-Butyl-4-methylpyridin-1-ium bromide (cas: 65350-59-6) belongs to pyridine derivatives. Pyridine’s the lone pair does not contribute to the aromatic system but importantly influences the chemical properties of pyridine, as it easily supports bond formation via an electrophilic attack. Pyridine groups exist in countless molecules, and their applications include catalysis, drug design, molecular recognition, and natural product synthesis.Reference of 65350-59-6